T-Cell Growth Factor Contains Activity that Supports Natural Killer Activity in Vitro

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Abstract
Lymphocytes from athymic and normal mice were tested and compared for cytotoxic activity after in vitro cultures supplemented with concanavalin A or T cell growth factor (TCGF). In addition to cytotoxic T cells, natural killer (NK) activity can be recovered from lymphocytes cultured in the presence of TCGF, but this is not the result of contaminating interferon in TCGF preparations. The NK nature of the effector cells was established by a panel of target cells [mouse mastocytoma P815 cells, lymphoma YAC cells, embryonal carcinoma Nulli-SCCl cells, lymphoma EL4 cells], including a teratocarcinoma tumor cell, which enabled the distinction of T cell- and NK cell-mediated lysis.