pH optimum of the reduction of dehydroascorbic acid by dithioerytritol
- 1 January 1993
- journal article
- research article
- Published by Taylor & Francis in Scandinavian Journal of Clinical and Laboratory Investigation
- Vol. 53 (4) , 367-371
- https://doi.org/10.3109/00365519309086629
Abstract
Ascorbic acid is a water soluble antioxidant, that is itself oxidized to dehydroascorbic acid. In order to detect dehydroascorbic acid by amperometri it has to be reduced to ascorbic acid beforehand. This reduction was performed with dithioerytritol at pH = 6.0 for lOmin, which is a choice between optimal reaction rate and stability of ascorbic acid. The limit of detection of ascorbic acid is 1.1 pmol making the assay useful for determination of ascorbic and dehydroascorbic acid in small tissue biopsies. Results of this method and a colorimetric method were comparable.Keywords
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