Hapten conjugation in the leucocyte migration inhibition test in allergic chromate eczema

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Abstract
The in vitro conjugation of Cr salts was studied, showing that trivalent Cr binds to protein 10 times more strongly than hexavalent Cr. The use of 2 different lymphocyte migration inhibition tests for the diagnosis of Cr allergy was also investigated. The capillary method proved superior to the Clausen technique, and potassium dichromate was shown to be a better antigen than chromium chloride. The test results correlated well with standard patch tests; the capillary method for the investigation of difficult cases of suspected Cr allergy is recommended.