A true microculture technique for human lymphocytes

Abstract
A true microculture technique utilizing only 0.01 ml of whole blood is presented. The blood is cultured in Eppendorf plastic microtubes in 1 ml of medium. This technique is easier, faster, and more economical than conventional methods. It is suitable for situations in which interphase cells or a small number (≤50) of metaphase cells are required.

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