Irreversible protein binding of acrylonitrile
- 1 January 1981
- journal article
- research article
- Published by Taylor & Francis in Xenobiotica
- Vol. 11 (1) , 51-56
- https://doi.org/10.3109/00498258109045271
Abstract
After i.p. injection of [2,3-14C]acrylonitrile [a toxic industrial chemical] to rats, a significant portion of radioactivity becomes irreversibly attached to proteins of liver, lung, spleen and other tissues. When rat liver microsomes were incubated with [2,3-14C]acrylonitrile, a time-dependent irreversible binding of radioactivity occurred to microsomal proteins. This binding was not dependent on NADPH. A high extent of binding to heat-inactivated microsomes indicated that no enzymic metabolic step was involved. The irreversible binding of [2,3-14C]acrylonitrile to rat liver microsomal protein in vitro was inhibited by thiols (cysteine, glutathione and mercaptoethanol). The greatest inhibitory potency was displayed by dithiocarb (diethyl dithiocarbamate).This publication has 11 references indexed in Scilit:
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