DNA‐BINDING PROTEIN AND THE CELL CYCLE INCRYPTOTHECODINIUM COHNII.I. ON THE RESOLUTION OF METABOLICALLY STABLE COMPONENTS1
- 1 September 1972
- journal article
- Published by Wiley in Journal of Phycology
- Vol. 8 (3) , 264-268
- https://doi.org/10.1111/j.1529-8817.1972.tb04038.x
Abstract
SUMMARY: A soluble protein extract, obtained after35S labeling exponential cultures ofCryptothecodinium cohniifor 4–5 generations, was fractionated on DNA‐cellulose. The protein eluted at high salt concentrations from this homologous DNA‐containing matrix was then separated, by chromatography on BioGel P150 and P200 sieve gels. Two prominent chromatographic species of 22,000 and 35,000 daltons plus a substantial amount of material in the molecular weight range of 80,000–140,000 were resolved. The application of this analytical procedure to the study of transcriptional control in the cell cycle is discussed.Keywords
This publication has 23 references indexed in Scilit:
- Nuclear Acidic Protein Changes during Differentiation in Physarum polycephalumScience, 1971
- DIVISION SYNCHRONY IN PRIMARY CULTURES OF AN ENDOZOIC DINOFLAGELLATE1Journal of Phycology, 1971
- RNA POLYMERASEAnnual Review of Biochemistry, 1971
- DIVISION SYNCHRONY IN PRIMARY CULTURES OF AN ENDOZOIC DINOFLAGELLATE1Journal of Phycology, 1971
- ELECTROPHORETIC IDENTITY OF POLYPEPTIDES FROM THE NUCLEAR MEMBRANE OF ANTHOPLEURA ‐ASSOCIATED ZOOXANTHELLAE1Journal of Phycology, 1971
- SOME PROPERTIES OF DNA FROM ZOOXANTHELLAE HARBORED BY AN ANEMONE ANTHOPLEURA ELEGANTISSIMA1Journal of Phycology, 1970
- Specific Binding of Rat Liver Nuclear Proteins to DNANature, 1970
- Studies on the RNA polymerase in Escherichia coli K12 using the mutation affecting its activityJournal of Molecular Biology, 1969
- RNA transcribed from reconstituted nucleoprotein is similar to natural RNAJournal of Molecular Biology, 1969
- Rearing the Colorless Marine Dinoflagellate Cryptothecodinium cohnii for Use as a Biochemical ToolThe Journal of Protozoology, 1968