Effect of 6-benzyladenine and Casein Hydrolysate on Micropropagation of Amorpha fruticosa

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Abstract
Using apical and axillary nodes as explants, a rapid and efficient method for propagation of Amorpha fruticosa L. has been developed. When grown on Murashige and Skoog (MS) medium supplemented with 8 mg dm−3 benzyladenine, 100 % explants responded with 4.94 shoots per explant after 6-weeks culture, and explants taken from the in vitro proliferated shoots subsequently produced multiple shoots when cultured on the same medium. The addition of casein hydrolysate (200 mg dm−3) enhanced the number of shoots up to 8.77 per subculture, and coconut milk was found to promote the shoot elongation and make them grow more vigorously, 82.53 % excised shoots were rooted on half-strength MS medium containing 2.0 mg dm−3 indoleacetic acid after 3 weeks of incubation. After acclimatization, all of the rooted plantlets established in soil, exhibiting uniform morphological and growth characteristics.