Incorporation of aerobic oxygen into the hydroxyglycyl intermediate during formation of C-terminal peptide amides by peptidylglycine α-amidating monooxygenase (PAM)
- 1 January 1991
- journal article
- research article
- Published by Royal Society of Chemistry (RSC) in Journal of the Chemical Society, Chemical Communications
- No. 8,p. 571-572
- https://doi.org/10.1039/c39910000571
Abstract
Incubation of D-phenylalanyl-L-phenylalanylglycine with peptidylglycine α-amidating monooxygenase (PAM) in the presence of 18O2 generates the corresponding hydroxyglycyl peptide whose FAB mass spectrum shows high incorporation of 18O into the hydroxy group; this suggests that the mechanism may involve direct hydroxylation of carbon.Keywords
This publication has 11 references indexed in Scilit:
- The 108-kDa peptidylglycine α-amidating monooxygenase precursor contains two separable enzymatic activities involved in peptide amidationBiochemical and Biophysical Research Communications, 1990
- A novel enzyme from bovine neurointermediate pituitary catalyzes dealkylation of .alpha.-hydroxyglycine derivatives, thereby functioning sequentially with peptidylglycine .alpha.-amidating monooxygenase in peptide amidationBiochemistry, 1990
- Copper-mediated oxidative C-terminal N-dealkylation of peptide-derived ligands. A possible model for enzymatic generation of desglycine peptide amidesJournal of the American Chemical Society, 1990
- Enzymatic peptidyl .alpha.-amidation proceeds through formation of an .alpha.-hydroxyglycine intermediateJournal of the American Chemical Society, 1989
- Investigations of polypeptide biosynthesis: formation of peptide amidesPublished by Walter de Gruyter GmbH ,1989
- Formation of peptide amides by peptidylglycine .alpha.-amidating monooxygenase: a new assay and stereochemistry of hydrogen lossJournal of the American Chemical Society, 1988
- Peptide α-AmidationAnnual Review of Physiology, 1988
- Biosynthesis of the C-terminal amide in peptide hormonesBioscience Reports, 1987
- N‐Acyl‐α‐iminosäure‐Derivate, III. Synthese von N‐Acyl‐α‐oxoaldiminenEuropean Journal of Organic Chemistry, 1986
- The amidating enzyme in pituitary will accept a peptide with c-terminal d-alanine as substrateBiochemical and Biophysical Research Communications, 1983