Cr 51-tagged red cell equilibration in dogs

Abstract
Equilibration of Cr51 -tagged red cells was studied in intact and chronically splenectomized dogs under control, hyper- and hypovolemic conditions, and in normovolemic hemorrhagic shock. Mixing of tagged cells in the circulation of control intact dogs was complete in 30 min., and in 10 min., in control splenectomized dogs. Simultaneous cell volume measurements with Fe59- and Cr51-tagged red cells were in agreement dur-inga 5-hr, period, suggesting that there was no appreciable elution of Cr51 from the red cells. Also, in dogs followed for 24 hr. cell volume was only 2% higher than that calculated from the 30-min. sample. Repeated determinations of cell volumes in control dogs varied less than 4%. Overtransfusion (93 ml/kg whole blood), hemorrhage to 50 mm Hg mean arterial pressure, hemorrhage to 50 mm Hg and reinfusion, or irreversible normovolemic hemorrhagic shock had no influence on the time of equilibration of Cr51 -tagged cells in the circulation of dogs during the 1st hr. To measure the red cell volume of the intact dog with the greatest accuracy, samples should be taken between 30 and 60 min. following injection of Cr51 -tagged red cells in the circulation.

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