Electron microscopic studies of macromolecules without appositional contrast

Abstract
Dedicated to the memory of Nicole Granboulan In pursuing electron microscopic studies of individual biomacromolecules without contrast-enhancing stains, conventional transmission electron microscopy was improved by the use of thin, large area carbon support films. Contrast of the bright-held appearance of the unstained macromolecules and complexes was strongly enhanced in a dark held obtained by insertion of a dark-held objective aperture or by tilting the condenser system. The results are exemplified by various biological macromolecules such as DNA-protein monolayers or ferritin molecules.

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