Collection of Human Oocytes for in Vitro Fertilization

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Abstract
By laparoscopy or laparotomy, oocytes were recovered from 42 (90%) of 47 patients. Follicles [185] containing 138 oocytes were aspirated by means of a specially constructed aspiration unit. The patients followed 2 different schemes pre-operatively. In the unstimulated patients an average of 1.3 pre-ovulatory oocytes per patient was collected, while treatment with 600-900 IU human menopausal gonadotropin + 6000-12,000 IU human chorionic gonadotropin resulted in an average of 2.0 pre-ovulatory oocytes per patient. Laparoscopy in combination with the described aspiration technique seems to be a clinically suitable method for collection of human oocytes for in-vitro fertilization.