Heteromeric nature of glucocorticoid receptor
- 1 January 1985
- journal article
- Published by Wiley in FEBS Letters
- Vol. 179 (1) , 138-142
- https://doi.org/10.1016/0014-5793(85)80208-8
Abstract
The wild‐type and a mutant receptor of S49.1 lymphoma cells have been shown by photoaffinity labelling to contain steroid‐binding polypeptides of M r 94 000 and 40 000, respectively. We investigated the molybdate‐stabilized forms of these receptors and obtained M r 325 000 and 285 000, respectively, by gel filtration and sedimentation analysis. Mild chymotrypsin treatment of the large wild‐type receptor resulted in a form of about M r 290 000 which contained a steroid‐binding polypeptide of M r 40 000. The data suggest that the high‐M r forms of glucocorticoid receptors are heteromeric in nature and contain one steroid‐binding polypeptide per complexKeywords
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