The active site and mechanism of action of bovine pancreatic ribonuclease. 5. The charge types at the active centre

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Abstract
(1) The activity of ribonuclease can be enhanced or depressed by solvents depending on the pH and the nature of the buffer. (2) A general method of determining the charge types of the groups at the active site of an enzyme, based on the use of organic solvents, is presented and applied to ribonuclease. (3) The pH-activity curve of ribonuclease has been measured in water 50% (v/v) dioxan and 50% (v/v) formamide in cationic-acid and in neutral-acid buffers. (4) The active site of ribonuclease contains two groups whose acid species are cationic.