Sulfated Glycoproteins Capable of Coagulating Calcium Carbonate Isolated from Pathological Human Bile*

Publisher Website
Google Scholar
Abstract
Pathological and normal human biles were fractionated centrifugally in the presence of salts. Of the resulting fractions, the major fraction (Fr. 2) obtained from pathological bile showed a strong coagulating effect on calcium carbonate suspension in water, whereas the fractions obtained from normal bile did not show any significant effect. Further fractionation on DEAE-Sephadex A-25 of Fr. 2 from the pathological bile afforded several subtractions. The major subtractions (Fr. 2–0.1 and Fr. 2–0.2) showed a very strong coagulating effect and were homogeneous on cellulose acetate electrophoresis. These fractions were characterized as sulfated glycoproteins by chemical and infrared spectral analyses. The present data together with previous observations suggest that sulfated glycoproteins are abnormal components in bile, responsible for gallstone formation.

This publication has 0 references indexed in Scilit: