Enzymatic Degradation of Lignin

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Abstract
The intermediates of biological degradation of lignin by Poria subacida (Peck) Sacc. were detected by paperchromatography. p-Hydroxy-m-methoxyphenylpyruvic acid was identified as its 2,4-dinitrophenylhydrazone in the methylenchloride extract of the culture filtrate of the medium, in which spruce native lignin was contained. Guaiacylglycerol-β-coniferylether and vanillic acid-like compound were recognized in acetone extracts and methylenchloride extract of the decayed spruce-wood meal. The decayed lignin extracted from the decayed wood-meal showed the remarkable variation and the existence of the more predominant amount of carbonyl groups, compared with native lignin on their ultraviolet absorption spectra and infrared absorption spectra, and gave no purple-red colour reaction with phloroglucin-hydrochloric acid reagent.