Evidence for expression of two distinct T cell receptor beta-chain transcripts in a human diphtheria toxoid-specific T cell clone.
Open Access
- 1 January 1988
- journal article
- research article
- Published by Oxford University Press (OUP) in The Journal of Immunology
- Vol. 140 (1) , 300-304
- https://doi.org/10.4049/jimmunol.140.1.300
Abstract
We have cloned and sequenced the human T cell receptor alpha- and beta-chains cDNA present in a lambda gt10 library derived from a human CD4+ diphtheria toxoid-specific T cell clone, PH28. Two 1.3-kb beta-transcripts within frame V, D, J joining were found. These two functional beta-transcripts use two members of different V beta-families, V beta 3 and V beta 4, one joined to D beta 1-1, J beta 1-1, C beta 1, the other joined to D beta 1-1, J beta 2-1, C beta 2. Moreover, we have cloned the two different V beta germ-line counterparts whose sequences confirm that these two V beta are not pseudogenes. Analysis of T cell receptor beta and alpha rearrangements in PH28 showed that a single rearrangement on each chromosome could be assigned either at the J beta or the J gamma loci. These results strongly suggest an absence of allelic exclusion for 1.3-kb mRNA beta-transcripts in a human antigen-specific T cell clone.This publication has 16 references indexed in Scilit:
- Organization and sequences of the diversity, joining, and constant region genes of the human T-cell receptor beta chain.Proceedings of the National Academy of Sciences, 1985
- Low frequency of somatic mutation in beta-chain variable region genes of human T-cell receptors.Proceedings of the National Academy of Sciences, 1985
- A Novel Mechanism of Somatic Rearrangement Predicted by a Human T-Cell Antigen Receptor β-Chain Complementary DNAScience, 1985
- Segregation of polymorphic T-cell receptor genes in human families.Proceedings of the National Academy of Sciences, 1985
- Analysis of cDNA clones specific for human T cells and the alpha and beta chains of the T-cell receptor heterodimer from a human T-cell line.Proceedings of the National Academy of Sciences, 1985
- Antigen-specific proliferative human T cell clones with specificity for diphtheria toxoid: genetic and molecular restriction by class II antigensEuropean Journal of Immunology, 1984
- The mouse T cell receptor: Structural heterogeneity of molecules of normal T cells defined by XenoantiserumCell, 1983
- The human T cell receptor: Appearance in ontogeny and biochemical relationship of α and β subunits on IL-2 dependent clones and T cell tumorsCell, 1983
- Aberrant rearrangements contribute significantly to the allelic exclusion of immunoglobulin gene expressionNature, 1981
- Activity of multiple light chain genes in murine myeloma cells producing a single, functional light chainCell, 1980