Neurotrophin induced cAMP and IP3 responses in PC12 cells
Open Access
- 14 June 1993
- journal article
- Published by Wiley in FEBS Letters
- Vol. 324 (2) , 147-152
- https://doi.org/10.1016/0014-5793(93)81382-a
Abstract
NGF and BDNF elevate cAMP and IP3 levels in membranes of PC12 cells within a subsecond time period. The cAM P formation induced by NGF and BDNF pretreatment for 2 s was reduced by GDP‐β‐S and PTX, but not the trkNGFR inhibitor K 252a. NGF, but not BDNF, induced IP3 formation. IP3 formation was reduced by K 252a, but not by GDP‐β‐S and PTX. Using p75NGFR expressing, but trkNGFR‐deficient PCNA cell membranes, NGF and BDNF induced cAMP formation, but not IP3 formation. We suggest that NGF and BDNF induced cAMP formation is mediated via a p75NGFR/G‐protein mediated mechanism, and IP3 formation via a K 252a sensitive pathway.Keywords
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