EFFECTS OF CALCIUM ON PROLIFERATION OF LACTIC STREPTOCOCCUS BACTERIOPHAGE II. ,

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Abstract
In a Ca-deficient, partially chemically defined medium, proliferation of 6 bacteriophage strains on their actively growing host bacteria could not take place unless soluble Ca was added. The various strains required different minimal concns. of Ca for optimal proliferation in this medium; these concns. ranged from 2.7 X 10-4 to 1.7 X 10-3 [image]. The stimulatory effect of Ca could not be explained by its effect upon adsorption, as under the exptl. conditions employed, 85, 89 and 75% of the particles of 3 bacteriophage strains studied were adsorbed during a 10 min. period in the absence of Ca, but no detectable bacteriophage proliferation occurred.