The Giemsa banding pattern of canine chromosomes, using a cell synchronization technique

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Abstract
Cytogenetic investigations of the domestic dog, Canis familiaris, were performed on one Doberman pinscher and two Boxer dogs. Conventional homogeneously stained and G-banded metaphases from peripheral blood lymphocyte cultures synchronized with amethopterin and bromodeoxyuridine were studied. These procedures permitted the unequivocal identification of all canine chromosomes. A canine chromosome idiogram was constructed on the basis of the G-banding pattern at the haploid 327-band resolution level. The secondary constrictions and tapering of the telomeric regions characteristic of several canine chromosomes are described. Q-, C-, and NOR-banding were also performed and the salient features are described. This karyotype should enhance the value of the canine species in cytogenetic investigations.Key words: canine karyotype, G-banding, Q-banding, C-banding, NOR-banding, cell synchronization idiogram.

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