The Presence of a Chromatin Boundary Appears to Shield a Transgene in Tobacco from RNA Silencing
Open Access
- 14 August 2003
- journal article
- Published by Oxford University Press (OUP) in Plant Cell
- Vol. 15 (9) , 2203-2217
- https://doi.org/10.1105/tpc.012070
Abstract
We present isogenic transgenic tobacco lines that carry at a given chromosomal position a β-glucuronidase (GUS) reporter gene either with or without the presence of the matrix-associated region known as the chicken lysozyme A element. Plants were generated with the Cre-lox site–specific recombination system using heterospecific lox sites. Analysis of GUS gene expression in plant populations demonstrates that the presence of the A element can shield against RNA silencing of the GUS gene. Protection was observed in two of three independent tobacco transformants. Plants carrying an A element 5′ of the GUS gene always had stable GUS activity, but upon removal of this A element, the GUS gene became silenced over time in two lines, notably when homozygous.Keywords
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