GA-68 LABELED RED-CELLS AND PLATELETS NEW AGENTS FOR POSITRON TOMOGRAPHY

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  • 1 January 1977
    • journal article
    • research article
    • Vol. 18  (6) , 558-562
Abstract
Red cells and platelets are labeled with 68Ga by preparing the 68Ga- hydroxy-quinoline (oxine) complex and mixing the complex with the separated cellular components. The complex is prepared by 1st breaking the 68Ga EDTA chelate with concentrated hydrochloric acid, adsorption of the Ga on a strong anionic ion-exchange column and elution of the Ga activity with distilled water. Oxine is added, the oxine complex extracted into chloroform, and, following evaporation and disollution, the solution is added to either red cells or platelets that were centrifuged from whole blood, washed and suspended in normal saline. After 15 min of incubation, labeling efficiencies of > 90% and yields of .apprx. 7 mCi are obtained from 18 mCi of 68Ga EDTA eluted from the generator. After injection of the Ga-labeled red cells in dogs, excellent blood pool images were obtained by positron tomography. The distribution corresponded to that of 11CO-labeled red cells. When labeled platelets were injected into dogs with experimentally induced intimal injury of on carotid artery, the area of injury was clearly visualized 40 min after tracer injection.