A Histone‐Specific Acetyltransferase is Associated with Simian‐Virus‐40 Chromatin

Abstract
[14C]Thymidine-labeled SV-40 nucleoprotein complexes were prepared from nuclei of lytically infected African green monkey kidney cells. The nucleoprotein complexes were further purified by sucrose gradient centrifugation and then incubated in the presence of [3H]acetyl-Co A. A transfer of [3H]acetyl groups to the endogenous histones H2B, H3 and H4 was observed. The enzyme was solubilized in the presence of 0.5 M NaCl and showed properties described for the DNA-binding acetyltransferase.