IDENTIFICATION OF CALCIUM-OXALATE CRYSTALS USING ALIZARIN RED-S STAIN

  • 1 January 1985
    • journal article
    • research article
    • Vol. 109  (2) , 186-189
Abstract
Calcium oxalate crystals stain with alizarin red S at a pH of 7.0 but not at a pH of 4.2. In contrast, calcium phosphate and calcium carbonate stain at a pH of both 7.0 and 4.2. This difference allows presumptive identification of calcium oxalate deposits. The identity of calcium oxalate can then be confirmed by its insolubility in 2 M acetic acid, since calcium carbonate and calcium phosphate are soluble. This procedure was applied for several yr. It is a rapid, reliable and technically simple procedure for distinguishing calcium oxalate from other calcium deposits.

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