Patterns of expression of the JIM4 arabinogalactan-protein epitope in cell cultures and during somatic embryogenesis in Daucus carota L.
- 1 January 1990
- journal article
- research article
- Published by Springer Nature in Planta
- Vol. 180 (2) , 285-292
- https://doi.org/10.1007/bf00194009
Abstract
Spatiotemporal patterns of expression of the cell-surface arabinogalactan-protein epitope defined by monoclonal antibody JIM4 (J.P. Knox et al., 1989, Development 106, 47–56) have been characterized by indirect immunofluorescence during the process of somatic embryogenesis in Daucus carota L. The JIM 4 epitope (J4e) occurred on cells established in culture from hypocotyl explants which appeared to derive, at least in part, from the epidermal cells of the hypocotyl. Cultures maintained in the presence of 2,4-dichlorophenoxyacetic acid developed proembryogenic masses of which only infrequent cells at the surface expressed J4e. Sub-culture at a low cell density and withdrawl of the synthetic auxin resulted in an increase in J4e expression in most surface cells and most abundantly in surface layers of cells at the future shoot end of developing embryos. The transition to heart-shaped embryos occurred concurrently with the expression of J4e by groups of cells beneath the developing cotyledons, at the junction of the future root and shoot. At this stage, J4e was also expressed by a single well-defined layer of cells at the surface of the embryos. Advancement to the mature torpedo stage was accompanied by the expression of the epitope on cells forming two regions of the future stele and of cells associated with the cotyledonary provascular tissue characteristic of the carrot seedling. At this stage there was substantially less expression of the marker antigen by epidermal cells, although infrequent expression by isolated cells of the epidermis was maintained. The correlation of J4e expression with the development and distinction of plant tissue patterns during somatic embryogenesis indicates a role for plasma-membrane arabinogalactan proteins in these processes.Keywords
This publication has 8 references indexed in Scilit:
- A family of abundant plasma membrane-associated glycoproteins related to the arabinogalactan proteins is unique to flowering plants.The Journal of cell biology, 1989
- Acquisition of embryogenic potential in carrot cell-suspension culturesPlanta, 1988
- Effect of 2,4-Dichlorophenoxyacetic Acid on the Expression of Embryogenic Program in CarrotPlant Physiology, 1986
- Identification and Isolation of Single Cells that Produce Somatic Embryos at a High Frequency in a Carrot Suspension CulturePlant Physiology, 1985
- A geometric analysis of somatic embryo formation in carrot cell culturesCanadian Journal of Botany, 1985
- Alternative Morphogenetic Events in Cell SuspensionsAmerican Journal of Botany, 1966
- Adventive Embryony in Tissue Cultures of the Wild Carrot, Daucus CarotaAmerican Journal of Botany, 1964
- Growth and Organized Development of Cultured Cells. II. Organization in Cultures Grown from Freely Suspended CellsAmerican Journal of Botany, 1958