Ligand-Conjugated Quantum Dots Monitor Antigen Uptake and Processing by Dendritic Cells
- 28 March 2007
- journal article
- Published by American Chemical Society (ACS) in Nano Letters
- Vol. 7 (4) , 970-977
- https://doi.org/10.1021/nl0700503
Abstract
The dendritic cell (DC) specific pathogen-uptake receptor (DC-SIGN) internalizes antigens for degradation and presentation onto MHC molecules. At the cell membrane, DC-SIGN forms nanoclusters that facilitate virus capture. However, internalized viruses, such as HIV-1, escape degradation. Here, we exploit ligand-conjugated, virus-sized, highly photostable quantum dots (QDs) to monitor in living cells antigen binding, entry, and trafficking. The antigen-coated QDs specific uptake and persistence in live DCs open the possibility for tracking antigen-presenting cells in vivo.Keywords
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