An Enzyme-linked Immunosorbent Assay (ELISA) for PBS Z1, a Defective Phage of Bacillus subtilis
- 1 September 1979
- journal article
- research article
- Published by Microbiology Society in Journal of General Virology
- Vol. 44 (3) , 741-746
- https://doi.org/10.1099/0022-1317-44-3-741
Abstract
The sensitivity, reproducibility and specificity of an enzyme-linked immunosorbent assay (ELISA) for the defective phage PBS Z1 of B. subtilis were investigated. Phages in concentrations between 108 and 2.5 .times. 1010 particles/ml could be assayed with this method. The coefficient of variation for concentrations between 5 .times. 108-5 .times. 109 particles/ml was about 10%. From other Bacillus phages tested, only the defective phages resembling PBS Z1 in morphology were detected efficiently with the ELISA for PBS Z1. ELISA is compared to other assays for PBS Z1.This publication has 5 references indexed in Scilit:
- The occurrence and taxonomic value of PBS X-like defective phages in the genus BacillusAntonie van Leeuwenhoek, 1978
- A simplification of the protein assay method of Lowry et al. which is more generally applicableAnalytical Biochemistry, 1977
- Enzyme-immunoassayClinica Chimica Acta; International Journal of Clinical Chemistry, 1977
- A counting method for determining the burst size of defective phages fromBacillus subtilisAntonie van Leeuwenhoek, 1977
- Properties of the defective phage of Bacillus subtilisJournal of Molecular Biology, 1968