INSITU LYMPHOID-CELLS OF MOUSE MAMMARY-TUMORS .2. CHARACTERIZATION OF LYMPHOID-CELLS SEPARATED FROM MOUSE MAMMARY-TUMORS

  • 1 January 1978
    • journal article
    • research article
    • Vol. 120  (6) , 1881-1886
Abstract
Lymphoid cells were isolated from mouse mammary tumors by isokinetic gradient centrifugation. .theta.-Bearing and ALS[anti-lymphocyte serum]-sensitive cells were always the largest subpopulations found in these tumors. In fractions 4-6 from the isokinetic gradient, .theta.-positive cells ranged from 8-46% in autochthonous tumors and 14-47% in passaged tumors, and ALS-positive cells ranged from 34-46% in autochthonous tumors and 14-100% in passaged tumors. SIg[surface immunoglobulin]-positive cells were only found in occasional tumors and, where present, averaged 2% of the separated population. Fc receptor-bearing cells averaged 15% and phagocytic cells averaged 8% of the cells found in fractions 4-6 of the gradient. Occasional ALS-positive and 1/3 of the Fc receptor-bearing and phagocytic cells also separated in fractions 7- of the isokinetic gradient. Although the individual tumors were very variable in the proportions of the various lymphoid subpopulations that they contained, this variability was a stable characteristic for at least 1 passage generation.

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