Identification of Nucleolus Organizer Chromosomes in Cattle (Bos TaurusL.) by Sequential Silver Staining + Rba Banding

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Abstract
A sequential «silver staining + RBA banding» procedure was applied to cytological preparations from six individuals of the Friesian breed of Bos taurus L. (2n = 60) for the identification of nucleolus organizer chromosomes. Chromosome identification followed the Reading system. Of 10 sequentially stained metaphase plates for each animal investigated, Ag-NORs were located on the telomeres of five pairs of autosomes identified as nos. 2, 3, 6, 11 and 27, indicating that two of the five NOR chromosomes in cattle have previously been misidentified.