Evidence for preferential repair of 3-carbethoxypsoralen plus UVA induced DNA lesions in the active MATα locus in Saccharomyces cerevisiae using the UvrABC assay
- 1 September 1993
- journal article
- research article
- Published by Oxford University Press (OUP) in Mutagenesis
- Vol. 8 (5) , 467-471
- https://doi.org/10.1093/mutage/8.5.467
Abstract
The occurrence of preferential repair in Saccharomyces cerevisiae of the active MATα locus compared with the inactive HMLα locus was confirmed after 254 nm UV irradiation. Experiments carried out using the UvrABC excinuclease assay with the monofunctional furocoumarin 3-carbethoxypsoralen (3-CPs) plus UVA radiation which induce mainly monoadducts in DNA demonstrated preferential repair of the active MATα locus compared with the inactive HMLα locus in a SIR+ strain. However, as after 254 nm UV irradiation, no difference in the rate of removal of 3-CPs plus UVA induced lesions was observed between the two loci in the sir-3 mutant in which both loci are active. Thus, it appears that 3-CPs plus UVA induced monoadducts as well as pyrimidine dinners a e subject to preferential repair.Keywords
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