An Evaluation of Serum "Trypsin" Tests
- 1 February 1962
- journal article
- research article
- Published by Oxford University Press (OUP) in Clinical Chemistry
- Vol. 8 (1) , 62-66
- https://doi.org/10.1093/clinchem/8.1.62
Abstract
When crystalline trypsin was dissolved at various concentrations in pooled serum, the rates of hydrolysis of α-benzoyl-1-arginineamide (BAA) and N-α-benzoyl-dl-arginine-β-naphthylamide (BANA) were not linear functions of the trypsin concentration. Also, the plot of trypsin activity in relation to trypsin concentration differed with different sera. The apparent tryptic activity toward these substrates is felt to be a function of the concentration of serum trypsin inhibitor and, with BANA only, some unidentifed proenzyme which is activated by trypsin. Therefore, it is concluded that it is not feasible to measure serum trypsin concentration with these substrates.Keywords
This publication has 4 references indexed in Scilit:
- Serum “Trypsin” (Or Arginine Exopeptidase) Screening Test for Cancer of the PancreasGastroenterology, 1960
- Trypsin Inhibitors of Human Serum. I. Standardization. Mechanism of Reaction, and Normal Values1Journal of Clinical Investigation, 1958
- STUDIES ON THE INHIBITION OF PROTEOLYTIC ENZYMES BY SERUMThe Journal of Experimental Medicine, 1952
- Studies of Various Tests for Malignant Neoplastic Diseases. VII. Serum Inhibitors of Chymotrypsin and TrypsinJNCI Journal of the National Cancer Institute, 1952