Cloning, nucleotide sequence determination and expression of the hyaluronate lyase gene

15 July 1995

journal article
Published by Oxford University Press (OUP) in FEMS Microbiology Letters

Vol. 130 (1) , 81-85
https://doi.org/10.1016/0378-1097(95)00187-a

Abstract

The Staphylococcus aureus 8325-4 hyaluronate lyase gene (hysA) was identified after detecting hyaluronate lyase activity expressed by phages from a genomic library. The hysA open reading frame, capable of encoding a protein of 91 980 Da, was identified by Tn5 mutagenesis and nucleotide sequencing. HysA shares 35 and 36% amino acid sequence identity with group B streptococcal hyaluronate lyase and pneumococcal hyaluronidase, respectively. A 94-kDa protein was expressed in Escherichia coli minicells, a result consistent with the coding capacity of hysA. Identification of the S. aureus 8325-4 hyaluronate lyase gene will allow the regulation of this putative virulence determinant to be studied.

Keywords

This publication has 0 references indexed in Scilit: