Preparative and Analytical Separation of Amygdalin and Related Compounds in Injectables and Tablets by Reversed-Phase HPLC and the Effect of Temperature on the Separation
- 1 March 1984
- journal article
- research article
- Published by Oxford University Press (OUP) in Journal of Chromatographic Science
- Vol. 22 (3) , 94-98
- https://doi.org/10.1093/chromsci/22.3.94
Abstract
Previous HPLC procedures for amygdalin and related compounds in injectables and tablets were either time consuming or produced inadequate separations of D-amygdalin and its epimer. A study of the effects of temperature on the separation resulted in development of an HPLC method for amygdalin and some related compounds, using water as the mobile phase at 15°C. Multimilligram quantities of amygdalin and related compounds were separated by this preparative procedure. The aqueous mobile phase allows the compounds to be recovered by simple lyophilization of the sample after elution. This permitted the carbon-13 NMR spectrum of the isolated aglyconic epimer of amygdalin to be reported for the first time. D-amygdalin, its L-mandelonitrile epimer (D-epiamygdalin), their hydrolysis products (the epimeric amides and epimeric acids), and the sugar gentiobiose were separated by the method.This publication has 1 reference indexed in Scilit:
- Analytical chemistry of amygdalinAnalytical Chemistry, 1978