Studies on the hydrogen belts of membranes: I. Diester, diether, and dialkyl phosphatidylcholines and polyoxyethylene glycerides in monolayers with cholesterol

Abstract
The hydrogen belts of membranes are defined as the regions consisting of hydrogen bond acceptors, i.e., the C=O groups of glycero- and sphingolipids, and hydrogen bond donors, i.e., cholesterol-OH, sphingolipid-OH, proteins, and water. Lipid-lipid hydrogen bonding in these belts has been suggested. The connection of such hypothetical bonding with the condensation effect, i.e., the apparent reduction of surface area occupied by phospholipids in mixed monolayers with cholesterol, has been tested with lipids possessing and lacking C=O groups: diester, diether, and dialkyl phosphatidylcholine, and analogous polyoxyethylene diglycerides. Condensation by cholesterol was observed for all lipids. Consequently, the hypothetical lipid-C=O-cholesterol hydrogen bonding is not a prerequisite for the condensation effect.

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