Structural Analysis of Differentiation Antigens Mol and Mo2 On Human Monocytes
- 1 January 1982
- journal article
- research article
- Published by Mary Ann Liebert Inc in Hybridoma
- Vol. 1 (3) , 329-337
- https://doi.org/10.1089/hyb.1.1982.1.329
Abstract
Mol and Mo2 are membrane differentiation antigens expressed by human monocytes, and in the case of Mo1, by granulocytes and null cells. Mo1 antigen expression is resistant to protease treatment of intact monocytes while Mo2 is protease sensitive; Mo2 antigenic activity is regenerated by trypsin-treated monocytes during culture under conditions that favor protein synthesis. Further biochemical analysis reveals that Mo1 is associated with a glycoprotein consisting of two subunits of 94,000 and 155,000 MW. Mo2 resides on a glycoprotein of 55,000 MW. Immunoprecipitation of Mo1- and Mo2-bearing structures from lysates of internally labeled cells indicates that these proteins are synthesized by human monocytes.Keywords
This publication has 14 references indexed in Scilit:
- Monoclonal antibody with specificity for monocytes and neuronsCell, 1981
- A rapid method for isolation of antigenically active human cell surface antigens associated with β2-microglobulin using a monoclonal antibodyJournal of Immunological Methods, 1981
- Characterization of T cell surface glycoproteins T1 and T3 present on all human peripheral T lymphocytes and functionally mature thymocytesEuropean Journal of Immunology, 1981
- Initial characterization of monoclonal antibodies against human monocytes.Proceedings of the National Academy of Sciences, 1980
- Macrophage heterogeneity in man. A subpopulation of HLA-DR-bearing macrophages required for antigen-induced T cell activation also contains stimulators for autologous-reactive T cells.The Journal of Experimental Medicine, 1980
- Production of monoclonal antibodies reacting with peripheral blood mononuclear cell surface differentiation antigens.The Journal of Immunology, 1980
- Mac‐1: a macrophage differentiation antigen identified by monoclonal antibodyEuropean Journal of Immunology, 1979
- Surface-specific iodination of membrane proteins of viruses and eucaryotic cells using 1,3,4,6-tetrachloro-3α,6α-diphenylglycolurilBiochemistry, 1978
- A Film Detection Method for Tritium‐Labelled Proteins and Nucleic Acids in Polyacrylamide GelsEuropean Journal of Biochemistry, 1974
- The Glomerular Permeability Determined by Dextran Clearance Using Sephadex Gel FiltrationScandinavian Journal of Clinical and Laboratory Investigation, 1968