Factors Affecting the Fertility of Frozen Bovine Spermatozoa

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Abstract
Fertility trials were conducted to determine the affect of buffers, spermatozoa concentrations and method of filling and sealing glass ampules. A total of 934, 945, 928, and 902 1st service cows were bred with semen extended and frozen in egg yolk-citrate, whole milk, yolk-glucose-glycine and skimmilk, respectively. The 75-day non-return rates were 64.7, 66.2, 41.3 and 65.3% respectively. Comparing 10, 20, and 30 million spermatozoa per insemination prior to freezing 469, 479, and 472 1st service cows resulted in a non-return rate of 63.1, 68.9 and 72.7% respectively. Comparing semen frozen in glass ampules that had been gravity-filled-tip-sealted; gravity-filled-cut, pulled-sealed; forced-filled-tip-sealed and force-filled-cut, pulled-sealed; 294, 275, 313, and 254 1st service cows resulted in a non-return rate of 69.0, 73.8, 63.9 and 70.5% respectively. These differences were significant.