Simple Microdilution Test for Detection of Metallo-β-Lactamase Production in Pseudomonas aeruginosa
Open Access
- 1 November 2002
- journal article
- research article
- Published by American Society for Microbiology in Journal of Clinical Microbiology
- Vol. 40 (11) , 4388-4390
- https://doi.org/10.1128/jcm.40.11.4388-4390.2002
Abstract
A microdilution test measuring imipenem MICs in the presence or absence of a mixture of EDTA plus 1,10-phenanthroline was developed and tested on 190 Pseudomonas aeruginosa isolates, including 18 VIM- and 4 IMP-type metallo-β-lactamase (MBL) producers. The chelator mixture reduced by fourfold or more the imipenem MICs for MBL producers, while a lower effect or no effect was usually observed with MBL nonproducers.Keywords
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