Chromatographic behavior of several mammalian tRNAs on acylated dihydroxyl-borate cellulose and Aminex A-28
Open Access
- 1 January 1977
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 4 (7) , 2191-2204
- https://doi.org/10.1093/nar/4.7.2191
Abstract
Studies of the chromatographic behavior of mammalian tRNAs from several sources, on acylated DBAE-cellulose indicate that species of tRNAASn, tRNAASP and tRNAHis can be retained on this matrix, while species of tRNATyr, tRNAAsn and tRNAAsp are not retained. Treatment of total rat liver tRNA with cyanogen bromide and subsequent chromatography on Aminex A-28 columns demonstrated that these tRNA species might contain Q (or Q*) nucleoside. However, comparable studies of the tRNA isolated from Walker 256 rat mammary tumor tissue demonstrated that this tumor tRNA almost totally lacks the hypermodified nucleosides Q and Q*. In addition, we have found that at least the major species of rat liver tRNAASn contains the Q nucleoside.Keywords
This publication has 20 references indexed in Scilit:
- Initial position of aminoacylation of individual Escherichia coli, yeast, and calf liver transfer RNAsBiochemistry, 1977
- Isolation of mammalian tRNAAspand tRNATyrby lectin-Sepharose affinity column chromatographyNucleic Acids Research, 1977
- Specific replacement of Q base in the anticodon of tRNA by guanine catalyzed by a cell-free extract of rabbit reticulocytesNucleic Acids Research, 1976
- Nucleotide sequence of human placenta cytoplasmic initiator tRNACell, 1975
- Structure of the modified nucleoside Q isolated from Escherichia coli transfer ribonucleic acid. 7-(4,5-cis-Dihydroxy-1-cyclopenten-3-ylaminomethyl)-7-deazaguanosineBiochemistry, 1975
- Chromatographic changes in specific tRNAs after reaction with cyanogen bromide and sodium periodateBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1974
- The absence of ribothymidine in specific eukaryotic transfer RNAS: I. Glycine and threonine tRNAS of wheat embryoBiochemical and Biophysical Research Communications, 1973
- The isolation and sequence analysis of transfer RNA The use of plaskon chromatography (RPC-5)Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1973
- Activity of a transfer RNA modifying enzyme during the development of Drosophila and its relationship to the su(s) locusJournal of Molecular Biology, 1973
- Selective inactivation of amino acid acceptor and ribosome-binding activities of Escherichia coli tRNA by modification with cyanogen bromideBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1971