Direct evidence for three 1,2-hydride migrations in the biosynthesis of cycloartenol from [2-13C2H3]acetate in tissue cultures of Physalis peruviana

Abstract
[13C2H]-Labelled cycloartenol (2) was isolated from cultured cells of Physalis peruviana fed with [2-13C2H3]acetate. The distribution of the deuterium atoms was determined by 13C-{1H}{2H} n.m.r. spectroscopy. The 1,2-hydride migrations (20-H from C-17, 17-H from C-13, and 8-H from C-9) were verified in the biosynthesis of cycloartenol (2).