Genetic analysis of temperature-sensitive mutants which define the gene for the major herpes simplex virus type 1 DNA-binding protein

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Abstract
Eight temperature-sensitive mutants of herpes simplex virus type 1 were assigned to complementation group 1-1. Members of this group fail to complement mutants in herpes simplex virus type 2 complementation group 2-2. The mutation of 1 member of group 1-1, tsHA1 of strain mP, was shown to map in or near the sequence which encodes the major herpes simplex virus type 1 DNA-binding protein. The mutations of 5 other members of group 1-1 map in or near the sequence in which the tsHA1 mutation maps, a sequence which lies near the center of UL [unique long region] between the genes for the viral DNA polymerase and viral glycoprotein gAgB. These mutants can be divided into 2 groups; the mutations of 1 group map between coordinates 0.385 and 0.398, and the mutations of the other group map between coordinates 0.398 and 0.413. At the nonpermissive temperature mutants in group 1-1 are viral DNA negative, and mutant-infected human laryngeal carcinoma HEp-2 cells fail to react with monoclonal antibody to the 130,000-dalton DNA-binding protein. Evidently, mutants in complementation groups 1-1 and 2-2 define the gene for the major herpes simplex virus DNA-binding protein, an early gene product required for viral DNA synthesis.