Two tissue-specific factors bind the erythroid promoter of the human porphobilinogen deaminase gene
Open Access
- 1 January 1989
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 17 (1) , 37-54
- https://doi.org/10.1093/nar/17.1.37
Abstract
We have studied the erythroid-specific promoter of the human gene coding for Porphobilinogen Deaminase (PBGD) by DNaseI footprintng, gel retardation and methylation interference assays. We show that this promoter, which is inducible during MEL cell differentiation, contains three binding sites for the erythroid-specific factor NF-E1 and one site for a second erythroid-specific factor, which we name NF-E2. NF-E1 is a factor that also binds the promoter and the enhancer (present in the 3' flanking region) of the human β-globin gene. NF-E2 has not yet been described and although it binds to a sequence containing the ApI consensus, it appears to be different from ApI.This publication has 33 references indexed in Scilit:
- Cooperativity and hierarchical levels of functional organization in the SV40 enhancerCell, 1988
- Position-independent, high-level expression of the human β-globin gene in transgenic miceCell, 1987
- In vitro binding of several cell-specific and ubiquitous nuclear proteins to the GT-I motif of the SV40 enhancer.Genes & Development, 1987
- Induction of a factor that binds to the polyoma virus A enhancer on differentiation of embryonal carcinoma cellsNature, 1987
- Purified transcription factor AP-1 interacts with TPA-inducible enhancer elementsCell, 1987
- Phorbol ester-inducible genes contain a common cis element recognized by a TPA-modulated trans-acting factorCell, 1987
- Nucleotide sequence of mouse 5-aminolevulinic acid synthase cDNA and expression of its gene in hepatic and erythroid tissuesGene, 1985
- DNA sequences required for regulated expression of β-globin genes in murine erythroleukemia cellsCell, 1984
- Three regions upstream from the cap site are required for efficient and accurate transcription of the rabbit β-globin gene in mouse 3T6 cellsCell, 1983
- Linkage of β-thalassaemia mutations and β-globin gene polymorphisms with DNA polymorphisms in human β-globin gene clusterNature, 1982