Measurement of Myeloid Cell Immune Suppressive Activity

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Abstract
This unit presents simple methods to assess the immunosuppressive properties of immunoregulatory cells of myeloid origin, such as myeloid‐derived suppressor cells (MDSCs), both in vitro and in vivo. These methods are general and could be adapted to test the impact of different suppressive populations on T cell activation, proliferation, and cytotoxic activity; moreover they could be useful to assess the influence exerted on immune suppressive pathways by genetic modifications, chemical inhibitors, and drugs. Curr. Protoc. Immunol. 91:14.17.1‐14.17.25. © 2010 by John Wiley & Sons, Inc.