PURIFICATION OF HUMAN MONOCYTES ON MICROEXUDATE-COATED SURFACES

  • 1 January 1978
    • journal article
    • research article
    • Vol. 120  (4) , 1372-1374
Abstract
Human blood monocytes, but not other Ficoll-Hypaque cells, adhere to plastic surfaces from which confluent BHK [baby hamster kidney] cells were removed. EDTA (3 mM) rapidly and completely removes monocytes, providing a simple technique for preparation of human monocytes in suspension. The method should be valuable for studies of monocyte function where monocyte monolayers are not suitable.