Effects of Varying Concentrations of Insulin Upon Protein Synthesis in Isolated Fat Cells

Abstract
Incorporation of 14C-amino acids by isolated epididymal adipose tissue cells into cell protein was determined. Rate of incorporation was proportional to duration of incubation. Fat cells demonstrated statistioally signifi-cant increased incorporation of amino acids into protein at physiologi-cal levels of insulin, the dose response curve being 10-800 [mu]U/ml. Insulin increased amino acid incorporation into fat cell protein in the absence of glucose.