INDEPENDENCE OF DEFICIENT EARLY GROWTH AND LATER REGRESSION OF (C57BL × 101)F2 MARROW GRAFTS IN (C57BL × 101)F1 HYBRID MICE

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Abstract
Marrow cells from individual (C57BL × 101) F2 donors were transplanted into irradiated (C57BL × 101 ) F2mice to determine whether or not two traits of C57BL marrow, namely, early deficient growth in H-2 heterozygotes and later regression of established grafts in F2 hybrids segregated independently in F1 mice. Marrow cells from F2 donors of H-2b/H-2b serotype failed to grow detectably in irradiated F2 mice as judged by the inability of the marrow to promote synthesis of DNA in recipient spleens 5 days after transplantation, when injected in doses of 5 × 105 nucleated cells; however, marrow cells from H-2b/H-2k and H-2b/H-2k F2 donors grew without apparent inhibition under identical conditions. The growth pattern of F2 marrow on transplantation into F2 mice remained correlated with its H-2 constitution even after prolonged (1 year) residence in primary F1 recipients, as judged on retrans plantation into secondary F1 test recipients. In contrast to these findings with 5 × 105 grafted cells, 10v nucleated marrow cells from H-2b/H-2b F2 donors were consistently able to repopulate the hemopoietic system in F2 mice. Moreover, the 12.5% of all F2 marrow grafts that regressed within 10 days after transplantation were distributed among the three segregating H-2 phenotypes. Significantly, 8 of 9 grafts of H-2b/H-2b serotype did not regress. It is concluded, therefore, that the genetic factors determining the initial growth of a C57BL marrow graft in an F1 hybrid do not determine the ultimate persistence or regression of an established C57BL marrow in an F1 hybrid.

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