Sensitive methods for determining subclasses of IgG anti‐A and anti‐B in sera of blood‐group‐O women with a blood‐group‐A or ‐B child

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Abstract
Summary: The determination of the subclasses of IgG antibodies against blood groups A and B is important in order to improve our understanding and predict haemolytic disease of the newborn due to IgG anti‐A or ‐B. We describe two techniques that circumvent the problem of the agglutination of A and B red cells by the corresponding IgG antibodies in saline: an antiglobulin consumption test and a modified solid‐phase micro‐immunofluorescence test. The results of the two techniques are compared with the results obtained in the indirect antiglobulin test beyond the saline agglutination titre in a microplate technique. The solid‐phase micro‐immunofluorescence test was the most sensitive for the determination of the subclasses of IgG anti‐A and ‐B. Usually sera contained IgG2 anti‐A, B in a higher titre than antibodies of other subclasses.