Radioimmunodetection of a Transplantable Human Bladder Carcinoma in a Nude Mouse
- 1 November 1984
- journal article
- research article
- Published by Wolters Kluwer Health in Investigative Radiology
- Vol. 19 (6) , 530-534
- https://doi.org/10.1097/00004424-198411000-00011
Abstract
The results of an in vitro mixed hemadsorption (MHA) assay predicted the success of in vivo tumor localization using a radio-iodinated, monoclonal, IgG1 antibody (A2) with reactivity to the human bladder carcinoma cell line RT4. In vitro, murine monoclonal antibodies A2 and G6, demonstrated reactivity to RT4 with titers of 1/1024 and 1/4 by MHA assay, respectively. In vivo results obtained with RT4 xenografts in athymic nude, Balb/c, mice indicated tumor uptakes of 1.10% dose/gram with A2 and 0.29% dose/gram with G6 at seven days after radiotracer injection. Successful scintigraphic imaging of tumor xenografts was achieved with A2 but not with G6 or radioiodinated mouse serum albumin.This publication has 9 references indexed in Scilit:
- Hybridoma Antibodies Reactive with Human Bladder Carcinoma Cell Surface AntigensJournal of Urology, 1983
- IMPROVED RADIOIMAGING AND TUMOR-LOCALIZATION WITH MONOCLONAL F(AB')21983
- Tumor Imaging with Radioactive Metal Chelates Conjugated to Monoclonal AntibodiesScience, 1982
- A spectrum of monoclonal antibodies reactive with human mammary tumor cells.Proceedings of the National Academy of Sciences, 1981
- Production of monoclonal antibodies in serum free mediumJournal of Immunological Methods, 1980
- Cell surface antigens of human malignant melanoma: definition of six antigenic systems with mouse monoclonal antibodies.Proceedings of the National Academy of Sciences, 1980
- THE PREPARATION OF 131I-LABELLED HUMAN GROWTH HORMONE OF HIGH SPECIFIC RADIOACTIVITYBiochemical Journal, 1963
- The in vivo localization of anti-Wagner-osteogenic-sarcoma antibodiesCancer, 1953
- PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENTJournal of Biological Chemistry, 1951