Protein, Esterase, and Peroxidase Patterns in the Phytophthora palmivora Complex from Cacao

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Abstract
Buffer-soluble, nonspecific protein and enzyme patterns from polyacrylamide disc gel electrophoresis were compared for 3 morphological forms (MF) and 1 other type of P. palmivora from cacao (Theobroma cacao) and for the type cultures of P. capsici and P. cinnamomi. Comparison of the total protein patterns indicated that each MF produced consistent major bands distinctly different from those of other MF. Clear differences between MF were also obtained in esterase patterns, but there were fewer differences in their peroxidase patterns. Protein and enzyme patterns from the type cultures of P. capsici and P. cinnamomi were strikingly different from each other and from those of most of the cacao isolates; some similarities were noted between MF4 and P. capsici. These results and other morphological studies indicate that the 3 MF of P. palmivora are sufficiently different to be classified as 3 distinct species.