STUDIES ON THE IMMUNOCHEMISTRY OF HUMAN LOW DENSITY LIPOPROTEINS UTILIZING AN HEMAGGLUTINATION TECHNIQUE

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Abstract
Hemagglutination is a specific and sensitive technique for investigating the purity of lipoproteins and the immunologic relationships between low density lipoprotein fractions. Sf 10-400 and Sf 3-9 lipoprotein fractions, isolated from human serum by a dextran sulfate-density gradient centrifugation procedure, appeared to contain only lipoprotein antigens and did not stimulate the production of antibodies against other serum proteins. Cross-absorption experiments with lipoproteins carried on "tanned" cells demonstrated that the Sf 3-9 lipoprotein fraction contains all the antigenic components of the Sf 10-400 lipoprotein fraction together with additional antigenic components not found in the Sf 10-400 lipoprotein fraction. Low density lipoproteins contain no antigens in common with the high density lipoproteins. An Sf 3-9 antiserum can be used to detect both Sf 3-9 and Sf 10-400 antigens. The Sf 3-9 lipoprotein fraction used as an antigen will detect antibodies against both Sf 3-9 and Sf 10-400 lipoprotein fractions.