Specific Purification of Lymphocyte Populations on a Digestible Immunoabsorbent

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Abstract
Summary: A new method utilizing an enzyme digestible immunoabsorbent has permitted fractionation and purification of lymphoid cells according to their surface properties. Spleen cells filtered through Sephadex anti-Fab columns were specifically depleted of immunoglobulin-bearing B cells. Passed cells, on the other hand, were enriched both in T and null staining cells. Ninety-eight per cent of the absorbed B cells could be recovered in a functionally viable state after solubilization of the immunoabsorbent with dextranase.