Biosynthesis of Pinguisone in an Axenic Culture of the LiverwortAneura pinguis

Abstract
Pinguisone accumulated in cultured gametophytes of Aneurapinguis to a significantly high level. A biosynthetic study on the formation of pinguisone was carried out by feeding [2-13C]-acetate to the cultured gametophytes. Pinguisone was labeled at an adequate level to determine the labeling positions by a 13C-NMR analysis. The labeling pattern indicated two-methyl migration and C-C bond cleavage of the main chain in farnesyl diphosphate in the formation of pinguisone.

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